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Validation of a high-performance liquid chromatography method for thiopurine S-methyltransferase activity in whole blood using 6-mercaptopurine as substrate.

Clinical chemistry and laboratory medicine - 25 Apr 2018

Rieger Hannah, Schmidt Patrik, Schaeffeler Elke, Abe Manabu, Schiffhauer Mira, Schwab Matthias, von Ahsen Nicolas, Zurek Gabriela, Kirchherr Hartmut, Shipkova Maria, Wieland Eberhard

Abstract excerpt

BACKGROUND: Variation in metabolism, toxicity and therapeutic efficacy of thiopurine drugs is largely influenced by genetic polymorphisms in the thiopurine S-methyltransferase (TPMT) gene. Determination of TPMT activity is routinely performed in patients to adjust drug therapy. METHODS: We further optimized a previously established high-performance liquid chromatography (HPLC) method by measuring TPMT activity in...

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Identifiers

PMID: 29194039
DOI: 10.1515/cclm-2017-0670

Genes

TPMT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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