Article
Development and validation of a reliable method for thiopurine methyltransferase (TPMT) enzyme activity in human whole blood by LC-MS/MS: An application for phenotypic and genotypic correlations.
Journal of pharmaceutical and biomedical analysis - 25 Oct 2017
Wiwattanakul Supaporn, Prommas Santirhat, Jenjirattithigarn Nuttawut, Santon Siwalee, Puangpetch Apichaya, Pakakasama Samart, Anurathapan Usanarat, Sukasem Chonlaphat
Abstract excerpt
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of thiopurine methyltransferase (TPMT) activity in human whole blood lysate, based on conversion of 6-mercaptopurine (6-MP) by TPMT to 6-methylmercaptopurine (6-MMP) using S-adenosyl-l-methionine (SAM) as the methyl donor. This method was improved from the previous laborious method for washing of red cell lysate...
Topics
- Chromatography, Liquid
- Genotype
- Humans
- Mercaptopurine
- Methyltransferases
- Phenotype
- Tandem Mass Spectrometry