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Article

Determination of Thiopurine S-Methyltransferase (TPMT) Activity in Human Erythrocytes by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Li Lie, Atkinson Natalya, Crews Kristine R, Molinelli Alejandro R

Abstract excerpt

Thiopurine methyltransferase (TPMT) is a cytosolic enzyme involved in the metabolism of thiopurine medications that are used in the treatment of multiple malignant and nonmalignant immunologic conditions. Polymorphisms in the TPMT gene associated with low enzyme activity can produce pronounced pharmacologic effects during therapy. The determination of TPMT erythrocyte activity is a valuable adjunct test to...

Topics

Humans
Chromatography, Liquid
Tandem Mass Spectrometry
Methyltransferases
Mercaptopurine
Genotype
Erythrocytes
S-Adenosylmethionine

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 38036847
DOI: 10.1007/978-1-0716-3541-4_43

Genes

TPMT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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Determination of Thiopurine S-Methyltransferase (TPMT) Activity in Human Erythrocytes by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).PMID 38036847Read ↗

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