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Article

Determination of thiopurine methyltransferase phenotype in isolated human erythrocytes using a new simple nonradioactive HPLC method.

Therapeutic drug monitoring - 1 Oct 2003

Indjova Dessislava, Shipkova Maria, Atanasova Srebrena, Niedmann Paul Dieter, Armstrong Victor W, Svinarov Dobrin, Oellerich Michael

Abstract excerpt

Genetic polymorphism of the S-methylation pathway catalyzed by thiopurine methyltransferase (TPMT) is responsible for variation in the metabolism, toxicity, and therapeutic efficacy of thiopurine drugs. This paper describe a new simple, nonradioactive HPLC method for determination of TPMT activity in isolated erythrocytes (Ery), based on the conversion of 6-mercaptopurine (pH 7.5, 37 degrees C) to...

Topics

Chromatography, High Pressure Liquid
Erythrocytes
Genotype
Humans
Methyltransferases
Phenotype
Radiochemistry

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 14508388
DOI: 10.1097/00007691-200310000-00014

Genes

TPMT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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Determination of thiopurine methyltransferase phenotype in isolated human erythrocytes using a new simple nonradioactive HPLC method.PMID 14508388Read ↗

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