Article
Thiopurine S-methyltransferase activity in human erythrocytes: a new HPLC method using 6-thioguanine as substrate.
European journal of clinical pharmacology - 1 May 1998
Kröplin T, Weyer N, Gutsche S, Iven H
Abstract excerpt
OBJECTIVES: To develop a non-radioactive assay to measure thiopurine S-methyltransferase (TPMT) activity. The assay was used to study the distribution of TPMT activity in a healthy German population. METHODS: The assay is based on the conversion of 6-thioguanine (6-TG) to 6-methylthioguanine (6-M...
Topics
- Alleles
- Chromatography, High Pressure Liquid
- Erythrocytes
- Female
- Genetic Variation
- Heterozygote
- Homozygote
- Humans
- Kinetics
- Male
- Methylation
- Methyltransferases
- Reproducibility of Results
- S-Adenosylmethionine
- Substrate Specificity
- Thioguanine