Article
Overexpression of RNase H partially complements the growth defect of an Escherichia coli delta topA mutant: R-loop formation is a major problem in the absence of DNA topoisomerase I.
Proceedings of the National Academy of Sciences of the United States of America - 11 Apr 1995
Drolet M, Phoenix P, Menzel R, Massé E, Liu L F, Crouch R J
Abstract excerpt
Previous biochemical studies have suggested a role for bacterial DNA topoisomerase (TOPO) I in the suppression of R-loop formation during transcription. In this report, we present several pieces of genetic evidence to support a model in which R-loop formation is dynamically regulated during trans...
Topics
- Cold Temperature
- DNA Topoisomerases, Type I
- DNA, Bacterial
- Escherichia coli
- Genetic Complementation Test
- Nucleic Acid Conformation
- Nucleic Acid Heteroduplexes
- Phenotype
- RNA, Bacterial
- Ribonuclease H
- Transcription, Genetic