Article
Chromosomal mapping and zygosity check of transgenes based on flanking genome sequences determined by genomic walking.
Experimental animals - 1 Apr 2004
Noguchi Akira, Takekawa Naho, Einarsdottir Thorbjorg, Koura Minako, Noguchi Yoko, Takano Kaoru, Yamamoto Yoshie, Matsuda Junichiro, Suzuki Osamu
Abstract excerpt
Transgenes can affect transgenic mice via transgene expression or via the so-called positional effect. DNA sequences can be localized in chromosomes using recently established mouse genomic databases. In this study, we describe a chromosomal mapping method that uses the genomic walking technique to analyze genomic sequences that flank transgenes, in combination with mouse genome database searches. Genomic DNA was...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.