Article
Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans.
RNA (New York, N.Y.) - 1 Apr 2006
Lee Rosalind C, Hammell Christopher M, Ambros Victor
Abstract excerpt
C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2,...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Caenorhabditis elegans
- Gene Silencing
- Mutation
- Oligonucleotide Probes
- RNA Interference