Article
The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site.
Cell - 26 Jan 1996
Bolland S, Kleckner N
Abstract excerpt
Nonreplicative transposition by Tn10/IS10 involves three chemical steps at each transposon end: cleavage of the two strands plus joining of one strand to target DNA. These steps occur within a synaptic complex comprising two transposon ends and monomers of IS10 transposase. We report four transpo...
Topics
- Amino Acid Sequence
- Binding Sites
- Catalysis
- Conserved Sequence
- DNA
- DNA Transposable Elements
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Protein Conformation
- Transposases