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Article

The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site.

Cell - 26 Jan 1996

Bolland S, Kleckner N

Abstract excerpt

Nonreplicative transposition by Tn10/IS10 involves three chemical steps at each transposon end: cleavage of the two strands plus joining of one strand to target DNA. These steps occur within a synaptic complex comprising two transposon ends and monomers of IS10 transposase. We report four transposase mutations that individually abolish each of the three chemical steps without affecting the synaptic complex. We...

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PMID: 8565068
DOI: 10.1016/s0092-8674(00)80977-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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