Article
Separate structural and functional domains of Tn4430 transposase contribute to target immunity.
Molecular microbiology - 1 Feb 2012
Lambin Michaël, Nicolas Emilien, Oger Cédric A, Nguyen Nathan, Prozzi Deborah, Hallet Bernard
Abstract excerpt
Like other transposons of the Tn3 family, Tn4430 exhibits target immunity, a process that prevents multiple insertions of the transposon into the same DNA molecule. Immunity is conferred by the terminal inverted repeats of the transposon and is specific to each element of the family, indicating that the transposase TnpA is directly involved in the process.However, the molecular mechanism whereby this protein...
Topics
- Amino Acid Substitution
- Bacillus thuringiensis
- DNA Transposable Elements
- Escherichia coli
- Genetic Techniques
- Mutation
- Transposases