Article
Characterization of lacZ complementation deletions using membrane receptor dimerization.
BioTechniques - 1 Feb 2003
Thormeyer D, Ammerpohl O, Larsson O, Xu Y, Asinger A, Wahlestedt C, Liang Z
Abstract excerpt
We describe the screening of lacZ deletions in mammalian cells and the discovery of a novel pair of lacZ deletions that can undergo alpha-complementation only when they are fused to peptides that interact with each other. The two lacZ deletions, delta N 11-75 and delta C 82-1023, were first characterized by fusing to two small interacting peptides and were then further analyzed by fusing to three membrane...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.