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Article

Correlation between catalytic efficiency and the transcription read-out in chemical complementation: a general assay for enzyme catalysis.

Biochemistry - 30 Mar 2004

Sengupta Debleena, Lin Hening, Goldberg Shalom D, Mahal Jacqueline J, Cornish Virginia W

Abstract excerpt

High-throughput assays for enzyme catalysis that can be applied to a broad range of chemical reactions are key to advances in directed evolution and proteomics. Recently, we reported such a general assay, chemical complementation, which links enzyme catalysis to reporter gene transcription in vivo using the yeast three-hybrid assay. In this proof-of-principle experiment, it was shown that a wild-type...

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PMID: 15035627
DOI: 10.1021/bi035810c

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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