Article
A novel selection system for recombinational and mutational events within an intron of a eucaryotic gene.
Nucleic acids research - 11 Sept 1990
Porter T, Pennington S L, Adair G M, Nairn R S, Wilson J H
Abstract excerpt
In order to identify a poison sequence that might be useful in studying illegitimate recombination of mammalian cell chromosomes, several DNA segments were tested for their ability to interfere with gene expression when placed in an intron. A tRNA gene and its flanking sequences (267 bp) were shown to inhibit SV40 plaque formation 100-fold, when inserted into the intron in the T-antigen gene. Similarly, when the...
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