Article
Site-directed mutations in Sindbis virus E2 glycoprotein's cytoplasmic domain and the 6K protein lead to similar defects in virus assembly and budding.
Virology - 1 Jul 1991
Gaedigk-Nitschko K, Schlesinger M J
Abstract excerpt
Site-directed mutagenesis was used to obtain four mutants with amino acid replacements in the cytoplasmic domain of the E2 glycoprotein and three with replacements in the 6K protein of Sindbis virus. All but one of these mutants yielded progeny virus after transfection of chicken embryo fibroblasts with RNA prepared by in vitro transcription of the virus cDNA; however, even this nonproducer mutant made virus...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Cells, Cultured
- Chick Embryo
- Cytoplasm
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed
- Phenotype
- RNA, Viral