Article
Disruption of cysteine-rich repeats of the p75 nerve growth factor receptor leads to loss of ligand binding.
The Journal of biological chemistry - 25 Jun 1991
Yan H, Chao M V
Abstract excerpt
Nerve growth factor (NGF) binds to a low affinity cell surface receptor (p75NGFR) which contains four extracellular repeats, rich in cysteine residues and negatively charged. We have made mutations in the receptor cDNA by inserting linkers in specific domains of the receptor. Nearly all the mutat...
Topics
- Affinity Labels
- Antibodies, Monoclonal
- Blotting, Western
- Chimera
- Cross-Linking Reagents
- Cysteine
- Humans
- Ligands
- Mutation
- Nerve Growth Factors
- Precipitin Tests
- Receptors, Cell Surface