Construction of a derivative of Tn917 containing an outward-directed promoter and its use in Bacillus subtilis.

doi:10.1099/00221287-137-1-107

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Article

Construction of a derivative of Tn917 containing an outward-directed promoter and its use in Bacillus subtilis.

Journal of general microbiology - 1 Jan 1991

Zagorec M, Steinmetz M

Abstract excerpt

Engineered variants of the transposon Tn917 have been widely used to obtain insertion mutations and transcriptional fusions in Bacillus subtilis and other Gram-positive bacteria. We have developed a novel Tn917-based methodology useful for isolation and characterization of mutants resulting from...

Topics

Bacillus subtilis
Cloning, Molecular
DNA Transposable Elements
DNA, Bacterial
Gene Expression
Genes, Bacterial
Mutation
Phenotype
Promoter Regions, Genetic
Transcription, Genetic
Transformation, Bacterial
beta-Galactosidase

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