Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of a derivative of Tn917 containing an outward-directed promoter and its use in Bacillus subtilis.

Journal of general microbiology - 1 Jan 1991

Zagorec M, Steinmetz M

Abstract excerpt

Engineered variants of the transposon Tn917 have been widely used to obtain insertion mutations and transcriptional fusions in Bacillus subtilis and other Gram-positive bacteria. We have developed a novel Tn917-based methodology useful for isolation and characterization of mutants resulting from gene over-expression. A Tn917 variant was constructed which contains a strong out-facing promoter near one end, able to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to DNA, Bacterial
Share to Gene Expression

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, Bacterial

Share to Mutation

Share to Phenotype

Share to Promoter Regions, Genetic

Share to Transcription, Genetic

Source

Identifiers

PMID: 1646272
DOI: 10.1099/00221287-137-1-107

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of a derivative of Tn917 containing an outward-directed promoter and its use in Bacillus subtilis.PMID 1646272Read ↗

Establishment of an arbitrary PCR for rapid identification of Tn917 insertion sites in Staphylococcus epidermidis: characterization of biofilm-negative and nonmucoid mutants.Semantic proximity Read ↗

A new mutation delivery system for genome-scale approaches in Bacillus subtilis.Semantic proximity Read ↗

Site-directed mutagenesis and gene deletion using reverse genetics.Semantic proximity Read ↗

Incidental mutations occur frequently during transposon mutagenesis.Semantic proximity Read ↗

Transposon-mediated random mutagenesis of Bacillus subtilis.Semantic proximity Read ↗

Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli.Semantic proximity Read ↗

Unravelling the genetic basis for competence development of auxotrophic Bacillus licheniformis 9945A strains.Semantic proximity Read ↗

Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.Semantic proximity Read ↗

Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome.Semantic proximity Read ↗

Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes.Semantic proximity Read ↗

Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production.Semantic proximity Read ↗

A novel engineering tool in the Bacillus subtilis toolbox: inducer-free activation of gene expression by selection-driven promoter decryptification.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.