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Article

Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli.

Journal of bacteriology - 1 Feb 1990

Guzzo J, Murgier M, Filloux A, Lazdunski A

Abstract excerpt

Pseudomonas virulence is thought to depend on multiple characteristics, including the production of an extracellular alkaline protease. We report the isolation, from a PAO1 DNA genomic bank, of a cosmid carrying the structural gene coding for alkaline protease. By in vivo mutagenesis using transposon Tn1735, which functions as a transposable promoter, the expression of an 8.8-kilobase DNA fragment under control...

Topics

Cloning, Molecular
DNA Transposable Elements
Endopeptidases
Escherichia coli
Genes, Bacterial
Genotype
Kinetics
Mutation
Phenotype
Promoter Regions, Genetic
Pseudomonas aeruginosa

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Proteins

Restriction Mapping

Serine Endopeptidases

beta-Galactosidase

Source

Open in PubMed Open full text

Identifiers

PMID: 2153662
DOI: 10.1128/jb.172.2.942-948.1990
PMCID: PMC208522

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2153662-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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