Article
Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production.
Journal of biotechnology - 31 May 2012
Borgmeier Claudia, Bongaerts Johannes, Meinhardt Friedhelm
Abstract excerpt
Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele--known to cause hypersecretion in Bacillus subtilis-- resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of...
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