Article
Establishment of an arbitrary PCR for rapid identification of Tn917 insertion sites in Staphylococcus epidermidis: characterization of biofilm-negative and nonmucoid mutants.
Applied and environmental microbiology - 1 Oct 2003
Knobloch Johannes K-M, Nedelmann Max, Kiel Kathrin, Bartscht Katrin, Horstkotte Matthias A, Dobinsky Sabine, Rohde Holger, Mack Dietrich
Abstract excerpt
Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, which were clustered...
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