Article
Isolation of a temperature-sensitive dnaA mutant of Staphylococcus aureus.
FEMS microbiology letters - 1 Jan 2006
Murai Nagamoto, Kurokawa Kenji, Ichihashi Norikazu, Matsuo Miki, Sekimizu Kazuhisa
Abstract excerpt
Of 750 temperature-sensitive mutants of Gram-positive Staphylococcus aureus, one was complemented by the dnaA gene. This mutant had a single base transition in the dnaA gene causing the amino-acid substitution mutation, Ala40Thr. Phage transduction experiments showed that this temperature-sensitive phenotype was linked with a drug-resistant marker inserted near the dnaA gene, suggesting the dnaA mutation is...
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