Article
Mutated polyadenylation signals for controlling expression levels of multiple genes in mammalian cells.
Biotechnology and bioengineering - 1 Mar 2009
Yang Yuansheng, Ho Steven C L, Yap Miranda G S
Abstract excerpt
A set of mutated SV40 early polyadenylation signals (SV40pA) with varying strengths is generated by mutating the AATAAA sequence in the wild-type SV40pA. They are shown to control the expression level of a gene over a 10-fold range using luciferase reporter genes in transient transfection assays. The relative strength of these SV40pA variants remains similar under three commonly used mammalian promoters and in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.