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Article

Thorough validation of siRNA-induced cell death phenotypes defines new anti-apoptotic protein.

Nucleic acids research - 23 Jan 2006

Wu Weilin, Hodges Emily, Höög Christer

Abstract excerpt

Loss-of-function by means of RNA interference in cultured human cells enables rapid pathway dissection on a genome-scale. Improved siRNA design and key validation protocols are required to eliminate falsely identified phenotypes resulting from potential off-target consequences. Here, we demonstrate a validation strategy involving several steps for verifying cell death phenotypes revealed during loss-of-function...

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Identifiers

PMID: 16432257
DOI: 10.1093/nar/gnj015
PMCID: PMC1345702

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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