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Article

Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the<i>Escherichia coli</i>Expression System

1 Jan 2006

Abstract excerpt

Two hundred thirteen cytochrome P450 (P450) genes were collected from bacteria and expressed based on an Escherichia coli expression system to test their hydroxylation ability to testosterone. Twenty-four P450s stereoselectively monohydroxylated testosterone at the 2alpha-, 2beta-, 6beta-, 7beta-, 11beta-, 12beta-, 15beta-, 16alpha-, and 17-positions (17-hydroxylation yields 17-ketoproduct). The hydroxylation...

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Identifiers

PMID: 16428858
DOI: 10.1271/bbb.70.307

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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