Article
A universal approach to the expression of human and rabbit cytochrome P450s of the 2C subfamily in Escherichia coli.
Archives of biochemistry and biophysics - 20 Oct 1995
Richardson T H, Jung F, Griffin K J, Wester M, Raucy J L, Kemper B, Bornheim L M, Hassett C, Omiecinski C J, Johnson E F
Abstract excerpt
Human cytochrome P450s 2C8, 2C9, 2C18, and 2C19 and rabbit cytochrome P450s 2C1, 2C2, 2C4, 2C5, and 2C16 were expressed from their respective cDNAs in Escherichia coli as chimeric enzymes in which a portion of the N-terminal membrane anchor sequence was replaced with a modified sequence derived f...
Topics
- Alleles
- Animals
- Aryl Hydrocarbon Hydroxylases
- Base Sequence
- Cloning, Molecular
- Cytochrome P-450 Enzyme System
- DNA, Complementary
- Escherichia coli
- Genetic Techniques
- Humans
- Molecular Sequence Data
- Rabbits
- Recombinant Fusion Proteins
- Steroid 16-alpha-Hydroxylase