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A universal approach to the expression of human and rabbit cytochrome P450s of the 2C subfamily in Escherichia coli.

Archives of biochemistry and biophysics - 20 Oct 1995

Richardson T H, Jung F, Griffin K J, Wester M, Raucy J L, Kemper B, Bornheim L M, Hassett C, Omiecinski C J, Johnson E F

Abstract excerpt

Human cytochrome P450s 2C8, 2C9, 2C18, and 2C19 and rabbit cytochrome P450s 2C1, 2C2, 2C4, 2C5, and 2C16 were expressed from their respective cDNAs in Escherichia coli as chimeric enzymes in which a portion of the N-terminal membrane anchor sequence was replaced with a modified sequence derived from P450 17A. For 2C1 and 2C2 removal of the extraneous 3'-untranslated sequence allowed the successful expression of...

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PMID: 7487078
DOI: 10.1006/abbi.1995.0013

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