Assay and properties of the mitochondrial dynamin related protein Opa1.

doi:10.1016/S0076-6879(05)04054-1

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Article

Assay and properties of the mitochondrial dynamin related protein Opa1.

Methods in enzymology - 1 Jan 2005

Griparic Lorena, van der Bliek Alexander M

Abstract excerpt

Opa1, also known as Mgm1 in yeast, is a mitochondrial member of the dynamin family. Unlike other dynamin family members, Opa1 has an N-terminal mitochondrial targeting sequence, suggesting that this protein is imported into mitochondria. Here, we describe biochemical techniques, such as mitochondrial isolation, digitonin extraction, a protease protection assay, and carbonate extraction, that were used to...

Topics

Animals
Brain
Cattle
Digitonin
GTP Phosphohydrolases
Guanosine Triphosphate
Humans
Mitochondria, Liver
Mitochondrial Proteins
Mutation
Protein Structure, Tertiary

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Open in PubMed

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PMID: 16413305
DOI: 10.1016/S0076-6879(05)04054-1

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