Article
HLA-DPB1 typing by PCR-SSO reverse dot blot hybridization after group-specific amplification.
Infusionstherapie und Transfusionsmedizin - 1 Dec 1994
Blasczyk R, Mohr M, Zimmermann R, Schwella N, Huhn D
Abstract excerpt
BACKGROUND: The allelic diversity of HLA-DPB1 antigens can be determined at the DNA level after PCR amplification. The pattern of polymorphism at the DPB1 locus makes it difficult to unambiguously assign all genotypes in a typing system using one single pair of generic primers. MATERIALS AND METH...
Topics
- Alleles
- Bone Marrow Transplantation
- Gene Amplification
- Genotype
- HLA-DP Antigens
- HLA-DP beta-Chains
- Heterozygote
- Histocompatibility Testing
- Humans
- Immunoblotting
- Leukemia
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Genetic
