Article
HLA-DPB1 typing by PCR-SSO reverse dot blot hybridization after group-specific amplification.
Infusionstherapie und Transfusionsmedizin - 1 Dec 1994
Blasczyk R, Mohr M, Zimmermann R, Schwella N, Huhn D
Abstract excerpt
BACKGROUND: The allelic diversity of HLA-DPB1 antigens can be determined at the DNA level after PCR amplification. The pattern of polymorphism at the DPB1 locus makes it difficult to unambiguously assign all genotypes in a typing system using one single pair of generic primers. MATERIALS AND METHODS: We apply here a simple technique based on the reverse dot blot analysis to the typing of HLA-DPB1 alleles. In...
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