Article
Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1993
Zwaal R R, Broeks A, van Meurs J, Groenen J T, Plasterk R H
Abstract excerpt
To understand how genotype determines the phenotype of the animal Caenorhabditis elegans, one ideally needs to know the complete sequence of the genome and the contribution of genes to phenotype, which requires an efficient strategy for reverse genetics. We here report that the Tc1 transposon ind...
Topics
- Alleles
- Animals
- Base Sequence
- Caenorhabditis elegans
- DNA
- DNA Transposable Elements
- Escherichia coli
- Freezing
- Gene Deletion
- Gene Expression Regulation
- Gene Library
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nematoda
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction