Article
Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi.
BMC developmental biology - 28 Dec 2005
Soares Miguel L, Haraguchi Seiki, Torres-Padilla Maria-Elena, Kalmar Tibor, Carpenter Lee, Bell Graham, Morrison Alastair, Ring Christopher J A, Clarke Neil J, Glover David M, Zernicka-Goetz Magdalena
Abstract excerpt
BACKGROUND: Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene silencing in late cleavage-stage mouse embryos that permits examination of phenotypes at post-implantation stages....
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