Article
Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast.
Proceedings of the National Academy of Sciences of the United States of America - 25 Aug 2015
Huang Mingtao, Bai Yunpeng, Sjostrom Staffan L, Hallström Björn M, Liu Zihe, Petranovic Dina, Uhlén Mathias, Joensson Haakan N, Andersson-Svahn Helene, Nielsen Jens
Abstract excerpt
There is an increasing demand for biotech-based production of recombinant proteins for use as pharmaceuticals in the food and feed industry and in industrial applications. Yeast Saccharomyces cerevisiae is among preferred cell factories for recombinant protein production, and there is increasing interest in improving its protein secretion capacity. Due to the complexity of the secretory machinery in eukaryotic...
Topics
- Genome, Fungal
- Microfluidics
- Mutation
- Saccharomyces cerevisiae Proteins