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Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast

14 Dec 2005

Abstract excerpt

The extracellular domain of epidermal growth factor receptor (EGFR-ECD) has been engineered through directed evolution and yeast surface display using conformationally-specific monoclonal antibodies (mAbs) as screening probes for proper folding and functional expression in Saccharomyces cerevisiae. An EGFR mutant with four amino acid changes exhibited binding to the conformationally-specific mAbs and human...

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PMID: 16355407
DOI: 10.1002/prot.20618

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