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Article

Using yeast surface display to engineer a soluble and crystallizable construct of hematopoietic progenitor kinase 1 (HPK1).

Acta crystallographica. Section F, Structural biology communications - 1 Jan 2021

Lau Wai L, Pearce Bradley, Malakian Heather, Rodrigo Iyoncy, Xie Dianlin, Gao Mian, Marsilio Frank, Chang Chiehying, Ruzanov Max, Muckelbauer Jodi K, Newitt John A, Lipovšek Daša, Sheriff Steven

Abstract excerpt

Hematopoietic progenitor kinase 1 (HPK1) is an intracellular kinase that plays an important role in modulating tumor immune response and thus is an attractive target for drug discovery. Crystallization of the wild-type HPK1 kinase domain has been hampered by poor expression in recombinant systems and poor solubility. In this study, yeast surface display was applied to a library of HPK1 kinase-domain variants in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33439152
DOI: 10.1107/S2053230X20016015
PMCID: PMC7805552

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