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Isolation of anti-T cell receptor scFv mutants by yeast surface display

1 Nov 1997

Abstract excerpt

Yeast surface display and sorting by flow cytometry have been used to isolate mutants of an scFv that is specific for the Vbeta8 region of the T cell receptor. Selection was based on equilibrium binding by two fluorescently labeled probes, a soluble Vbeta8 domain and an antibody to the c-myc epitope tag present at the carboxy-terminus of the scFv. The mutants that were selected in this screen included a scFv with...

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PMID: 9514119
DOI: 10.1093/protein/10.11.1303

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