Article
Development of a novel strategy for engineering high-affinity proteins by yeast display.
Protein engineering, design & selection : PEDS - 1 Jun 2006
Richman S A, Healan S J, Weber K S, Donermeyer D L, Dossett M L, Greenberg P D, Allen P M, Kranz D M
Abstract excerpt
Yeast display provides a system for engineering high-affinity proteins using a fluorescent-labeled ligand and fluorescence-activated cell sorting (FACS). In cases where it is difficult to obtain purified ligands, or to access FACS instrumentation, an alternative selection strategy would be useful. Here we show that yeast expressing high-affinity proteins against a mammalian cell surface ligand could be rapidly...
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