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Article

Improved mutants from directed evolution are biased to orthologous substitutions.

Protein engineering, design & selection : PEDS - 1 Jun 2006

Cochran Jennifer R, Kim Yong-Sung, Lippow Shaun M, Rao Balaji, Wittrup K Dane

Abstract excerpt

We have engineered human epidermal growth factor (EGF) by directed evolution through yeast surface display for significantly enhanced affinity for the EGF receptor (EGFR). Statistical analysis of improved EGF mutants isolated from randomly mutated yeast-displayed libraries indicates that mutations are biased towards substitutions at positions exhibiting significant phylogenetic variation. In particular, mutations...

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Source

Identifiers

PMID: 16740523
DOI: 10.1093/protein/gzl006

Genes

EGF
EGFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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