Article
In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.
Gene - 11 Jul 2001
Danielsen S, Eklund M, Deussen H J, Gräslund T, Nygren P A, Borchert T V
Abstract excerpt
The 'detergent lipase' Lipolase, from Thermomyces lanuginosa was subjected to a combinatorial protein engineering/phage display approach with the aim of identifying new enzyme variants with improved characteristics in the presence of detergents. First it was demonstrated that wild-type Lipolase could be produced in Escherichia coli retaining full activity and be displayed as an active enzyme fused to coat protein...
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