In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.

doi:10.1016/s0378-1119(01)00527-3

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Article

In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.

Gene - 11 Jul 2001

Danielsen S, Eklund M, Deussen H J, Gräslund T, Nygren P A, Borchert T V

Abstract excerpt

The 'detergent lipase' Lipolase, from Thermomyces lanuginosa was subjected to a combinatorial protein engineering/phage display approach with the aim of identifying new enzyme variants with improved characteristics in the presence of detergents. First it was demonstrated that wild-type Lipolase could be produced in Escherichia coli retaining full activity and be displayed as an active enzyme fused to coat protein...

Topics

Bacteriophages
Computer Graphics
Escherichia coli
Gene Library
Genetic Variation
Lipase
Mitosporic Fungi
Models, Molecular

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PMID: 11470533
DOI: 10.1016/s0378-1119(01)00527-3

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