Article
Activation defects caused by mutations in Escherichia coli rpoA are promoter specific.
Journal of bacteriology - 1 Aug 1992
Gussin G N, Olson C, Igarashi K, Ishihama A
Abstract excerpt
Escherichia coli RNA polymerases containing mutated alpha subunits were tested for their ability to respond to three different positive regulators (activators) in vitro. The two alpha (rpoA) mutants, alpha-256 and alpha-235, have deletions of the C-terminal 73 and 94 amino acids, respectively. In runoff transcription assays catalyzed by reconstituted holoenzyme, the effects of the mutations on each of three...
Topics
- Binding Sites
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Enzymologic
- Mutation
- Promoter Regions, Genetic
- RNA Cap-Binding Proteins
- RNA-Binding Proteins