Article
Functional map of the alpha subunit of Escherichia coli RNA polymerase: insertion analysis of the amino-terminal assembly domain.
Journal of molecular biology - 12 May 1995
Kimura M, Ishihama A
Abstract excerpt
The alpha subunit of Escherichia coli RNA polymerase plays a key role in assembly of the core enzyme. Deletion analysis of alpha indicated that the amino-terminal domain consisting of 215 amino acid residues between positions 21 and 235 is involved in this assembly. For fine mapping of the site(s) within this region required for subunit-subunit contacts, we constructed a set of insertion mutants of the rpoA gene,...
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