Article
A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase.
Nucleic acids research - 25 Jun 1990
Mehrpouyan M, Champney W S
Abstract excerpt
A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min. on the bacterial chromosome. The rate of RNA synthesis in vivo and the accumulation of ribosomal RNA were significantly reduced in the mutant at 44 degrees C. The...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genetic Complementation Test
- Kinetics
- Mutation
- Operon
- Temperature
- Templates, Genetic
- Transcription, Genetic