Article
Escherichia coli rpoA mutation which impairs transcription of positively regulated systems.
Molecular microbiology - 1 Nov 1991
Thomas M S, Glass R E
Abstract excerpt
The rpoA341 (phs) mutation of Escherichia coli results in decreased expression of several positively regulated operons and has been mapped to within or very near the rpoA gene encoding the alpha subunit of RNA polymerase. We have shown that plasmid-directed synthesis of the wild-type alpha subunit can complement the defective phenotypes associated with this mutation consistent with its proposed location within...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- DNA Mutational Analysis
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Operon
- Phenotype
- RNA Polymerase I
- Recombinant Fusion Proteins