Article
Mutations in β' subunit of Escherichia coli RNA polymerase perturb the activator polymerase functional interaction required for promoter clearance.
Molecular microbiology - 1 Jun 2011
Swapna Ganduri, Chakraborty Atanu, Kumari Vandana, Sen Ranjan, Nagaraja Valakunja
Abstract excerpt
Transcription activator C employs a unique mechanism to activate mom gene of bacteriophage Mu. The activation process involves, facilitating the recruitment of RNA polymerase (RNAP) by altering the topology of the promoter and enhancing the promoter clearance by reducing the abortive transcription. To understand the basis of this multi-step activation mechanism, we investigated the nature of the physical...
Topics
- Amino Acid Motifs
- Bacteriophage mu
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
- Binding Sites
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Viral
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- Trans-Activators
- Transcription, Genetic
- Transcriptional Activation