Article
Multiple mutant of Escherichia coli synthesizing virtually thymineless DNA during limited growth.
Journal of bacteriology - 1 Jul 1992
el-Hajj H H, Wang L, Weiss B
Abstract excerpt
The dut gene of Escherichia coli encodes deoxyuridine triphosphatase, an enzyme that prevents the incorporation of dUTP into DNA and that is needed in the de novo biosynthesis of thymidylate. We produced a conditionally lethal dut(Ts) mutation and isolated a phenotypic revertant that had a mutation in an unknown gene tentatively designated dus (for dut suppressor). The dus mutation restored the ability of the dut...
Topics
- DNA Glycosylases
- DNA Repair
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genes, Suppressor
- Genotype
- Kinetics
- Mutation
- N-Glycosyl Hydrolases
- Species Specificity
- Thymidine