Article
dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae.
Nucleic acids research - 1 Jan 2006
Guillet Marie, Van Der Kemp Patricia Auffret, Boiteux Serge
Abstract excerpt
We identified a viable allele (dut1-1) of the DUT1 gene that encodes the dUTPase activity in Saccharomyces cerevisiae. The Dut1-1 protein possesses a single amino acid substitution (Gly82Ser) in a conserved motif nearby the active site and exhibits a greatly reduced dUTPase activity. The dut1-1 single mutant exhibits growth delay and cell cycle abnormalities and shows a strong spontaneous mutator phenotype. All...
Topics
- Alleles
- Amino Acid Sequence
- Amino Acid Substitution
- DNA Damage
- DNA, Fungal
- Gene Deletion
- Genomic Instability
- Molecular Sequence Data
- Mutation
- Pyrophosphatases
- Saccharomyces cerevisiae