Article
Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling.
Nature communications - 13 Apr 2018
Oldrini Barbara, Curiel-García Álvaro, Marques Carolina, Matia Veronica, Uluçkan Özge, Graña-Castro Osvaldo, Torres-Ruiz Raul, Rodriguez-Perales Sandra, Huse Jason T, Squatrito Massimo
Abstract excerpt
To accurately recapitulate the heterogeneity of human diseases, animal models require to recreate multiple complex genetic alterations. Here, we combine the RCAS-TVA system with the CRISPR-Cas9 genome editing tools for precise modeling of human tumors. We show that somatic deletion in neural stem cells of a variety of known tumor suppressor genes (Trp53, Cdkn2a, and Pten) leads to high-grade glioma formation....
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