Article
Single nucleotide polymorphism analysis based on minisequencing coupled with a fluorescence microsphere technology.
Journal of nanoscience and nanotechnology - 1 Aug 2005
Li Zheng-Ping, Kambara Hideki
Abstract excerpt
In this paper, we describe a new method for detection of single nucleotide polymorphisms (SNPs) by applying the minisequencing principle to a fluorescence microsphere format. The specific primer, which was designed to anneal to its target of genomic DNA fragment immediately upstream of the polymorphic site, was immobilized to carboxylated Luminex microspheres as a probe. The primer was hybridized with genomic DNA...
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