Article
From gels to chips: "minisequencing" primer extension for analysis of point mutations and single nucleotide polymorphisms.
Human mutation - 1 Jan 1999
Syvänen A C
Abstract excerpt
In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allo...
Topics
- Amino Acid Substitution
- DNA Mutational Analysis
- DNA Primers
- DNA-Directed DNA Polymerase
- Electrophoresis, Agar Gel
- Enzyme-Linked Immunosorbent Assay
- Genotype
- Humans
- Nucleotides
- Point Mutation
- Polymerase Chain Reaction
- Sensitivity and Specificity