Article
From gels to chips: "minisequencing" primer extension for analysis of point mutations and single nucleotide polymorphisms.
Human mutation - 1 Jan 1999
Syvänen A C
Abstract excerpt
In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allows highly specific detection of point mutations and single nucleotide polymorphisms (SNPs). Because all SNPs can be...
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