Minisequencing: a specific tool for DNA analysis and diagnostics on oligonucleotide arrays.

doi:10.1101/gr.7.6.606

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Article

Minisequencing: a specific tool for DNA analysis and diagnostics on oligonucleotide arrays.

Genome research - 1 Jun 1997

Pastinen T, Kurg A, Metspalu A, Peltonen L, Syvänen A C

Abstract excerpt

We describe a method for multiplex detection of mutations in which the solid-phase minisequencing principle is applied to an oligonucleotide array format. The mutations are detected by extending immobilized primers that anneal to their template sequences immediately adjacent to the mutant nucleot...

Topics

DNA-Directed DNA Polymerase
Genetic Carrier Screening
Genetic Variation
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction
RNA
Sequence Analysis, DNA
Sequence Analysis, RNA

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