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Parallel analysis of mutant human glucose 6-phosphate dehydrogenase in yeast using PCR colonies.

Biotechnology and bioengineering - 5 Dec 2005

Merritt Joshua, Butz James A, Ogunnaike Babatunde A, Edwards Jeremy S

Abstract excerpt

We demonstrate a highly parallel strategy to analyze the impact of single nucleotide mutations on protein function. Using our method, it is possible to screen a population and quickly identify a subset of functionally interesting mutants. Our method utilizes a combination of yeast functional complementation, growth competition of mutant pools, and polymerase colonies. A defined mutant human...

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PMID: 16193512
DOI: 10.1002/bit.20726

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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