Article
A streamlined process to phenotypically profile heterologous cDNAs in parallel using yeast cell-based assays.
Genome research - 1 Nov 2001
Tugendreich S, Perkins E, Couto J, Barthmaier P, Sun D, Tang S, Tulac S, Nguyen A, Yeh E, Mays A, Wallace E, Lila T, Shivak D, Prichard M, Andrejka L, Kim R, Melese T
Abstract excerpt
To meet the demands of developing lead drugs for the profusion of human genes being sequenced as part of the human genome project, we developed a high-throughput assay construction method in yeast. A set of optimized techniques allows us to rapidly transfer large numbers of heterologous cDNAs from nonyeast plasmids into yeast expression vectors. These high- or low-copy yeast expression plasmids are then converted...
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