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The PcrA3 mutant binds DNA and interacts with the RepC initiator protein of plasmid pT181 but is defective in its DNA helicase and unwinding activities.

Plasmid - 1 Sept 2005

Anand Syam P, Chattopadhyay Anasuya, Khan Saleem A

Abstract excerpt

Plasmid rolling-circle replication initiates by covalent extension of a nick generated at the plasmid double-strand origin (dso) by the initiator protein. The RepC initiator protein binds to the plasmid pT181 dso in a sequence-specific manner and recruits the PcrA helicase through a protein-protein interaction. Subsequently, PcrA unwinds DNA at the nick site followed by replication by DNA polymerase III. The...

Topics

Adenosine Triphosphatases
Bacterial Proteins
Base Sequence
DNA Helicases
DNA, Bacterial
Genetic Complementation Test
Histidine
Molecular Sequence Data
Mutation
Plasmids
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Staphylococcus aureus

Source

Identifiers

PMID: 16122559
DOI: 10.1016/j.plasmid.2005.01.004

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The PcrA3 mutant binds DNA and interacts with the RepC initiator protein of plasmid pT181 but is defective in its DNA helicase and unwinding activities.PMID 16122559Read ↗

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