Article
The A184V missense mutation of the dnaA5 and dnaA46 alleles confers a defect in ATP binding and thermolability in initiation of Escherichia coli DNA replication.
Molecular microbiology - 1 Jun 1996
Carr K M, Kaguni J M
Abstract excerpt
The temperature-sensitive dnaA5 and dnaA46 alleles each contain two missense mutations. These mutations have been separated and the resulting mutant proteins studied with regard to their role in initiation of DNA replication in vitro. Whereas the His-252 to tyrosine substitution (H252Y) unique to...
Topics
- Adenosine Triphosphate
- Alleles
- Bacterial Proteins
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genetic Complementation Test
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Mutagenesis
- Point Mutation
- Replication Origin
- Structure-Activity Relationship
- Temperature